News & Views
GSBTM Project Investigator Dr. C. G. Joshi receives research of Grant of Rs 40.85 lakh from Department of Biotechnology, Government of India.
GSBTM congratulates the GSBTM Project Investigators for Promotions
GSBTM Investigator and GSBTM research team at Department of Biosciences, Saurashtra University developed a Software for measuring leaf area of plants
Research Note: Anther culture – A Promising Area in the field of Plant tissue culture research
GSBTM is happy to congratulate GBRCP participants for getting admission in PG programmes
GSBTM Project Investigator Dr. C. G. Joshi receives research of Grant of Rs 40.85 lakh from Department of Biotechnology, Government of India.

GOI New Delhi has sanctioned one research project on "Differential gene expression in udder of buffalo in induced lactation model" budget Rs.40.85 lakh. PI C. G. Joshi

Project Title: “Differential gene expression in udder of buffalo in induced lactation model”
Principal Investigator: Dr. C. G. Joshi
Project Grant: DBT GOI
Budget: Rs 40.85 Lakh
Project Summary: Milk production is a complex trait involving several systems and influenced by several genetic and non-genetic factors. Being polygenic in nature, large many genes are involved in the process of milk production, however their nature and role in the process is not fully elucidated. Since there is no direct access/knowledge about the milk trait genes, many scientists resorted to the marker-assisted selection based on microsatellite markers. To achieve this, a very dense genetic map of polymorphic microsatellite needs to be developed, and the process is very time consuming, resource demanding. Ideally 3000, or atleast 300 polymorphic microsatellites need to be mapped for very dense or average density map. These microsatellites are to be typed on a pedigreed herd with wide variation in milk yield to identify microsatellite marker that are tightly linked to milk trait, in turn these microsatellite markers will be used for marker assisted selection. But these will not be of any significant help in direct manipulation of milk genes. The process is exhaustive and results are not encouraging. On the other hand a direct approach that is aimed at targeting genes involved in milk production, rather than looking for any linked markers could be more rewarding. Being complex process and the trait has least significance in human; milk production’s physiology is not studied in depth at molecular level, except the knowledge of hormonal control of lactation. An experiment was conducted at this institute to know the genes expressed or switched off during lactation, using differential display technique, in udder tissue of heifer, lactating and dry buffalo and a research project is in progress to investigate it further in depth. However, induced lactation model will more closely examine the genes involved in the lactogenesis and thus –eliminating contamination of genes involved in mammogenesis. Suppressive subtractive hybridization technique is more robust and minimizes false positive hence selected for the proposed project. The ultimate aim of the project is to generate udder specific ESTs library which will be plotted on chip to develop “Dugdhpatri” which will have genes identified into groups like mammogenesis, lactogenesis, increase milk production, milk composition. Informative polymorphism in these genes, if at all are there will be identified and will be used for animal selection.

 Congratulation on promotions
GSBTM Project Investigator Dr. J. V. Solanki is promoted as Principal and Dean, Veterinary College,AAU,
Anand. He has 9 publication in different Journals like Indian Journal of Poultry Science; XXII World Poultry Congress, Turkey; Indian Journal of Animal Science; Buffalo Journal; Indian Journal of Veterinary Science. He has handled various DBT projects and National level Coordinate Projects and State level Projects.

Dr. C. G. Joshi is promoted as Professor in Animal Biotechnology.
Dr. C. G. Joshi has 11 publication in different Journals like Indian Journal of Poultry Science; XXII World Poultry Congress, Turkey; Indian Journal of Animal Science; Buffalo Journal; Indian Journal of Veterinary Science; Pashudhan. He has handled various DBT projects and National level Coordinate Projects and State level Projects.
GSBTM Project Investigator Dr. D. R. Patel is promoted as Professor in Agri. Biotechnology.
He has a publication of 12 research paper in International (2) and National Journals (10); 42 paper presentation at Seminar / Symposium. He hold membership in Indian Society of Oilseeds Research; Horticulture society of Gujarat; GAU Research Council - Plant Breeding Genetics subcommittee; Gujarat Association for Agricultural Sciences; Centre for Research and Information on Low-External input and sustainable Agriculture, Netherlands; Biotechnology and development monitor, Netherlands; International network for improvement of banana and plantain.
GSBTM Investigator and GSBTM research team at Department of Biosciences, Saurashtra University developed a Software for measuring leaf area of plants
GSBTM Investigator Dr. Vrinda Thaker and a research team at Saurashtra University, recently developed a software for Leaf Area measurement. Small leaf area, of the leaflets of plants such as Acacia nilotica and Mimosa pudica (‘Touch me not plant’) can be measured accurately which is otherwise not possible by conventional methods. The sensitivity of the software developed is upto is 0.035 mm2. Conventional methods have various drawbacks such as inaccuracy of measurement and are also time consuming.

The software developed is very accurate and rapid. Indigenously developed leaf area meter is not available in market at present. Imported leaf area meters are quite sensitive but very expensive. This software requires a digital camera or a scanner to take images and once images are transferred to personal computer or Laptop, this software measure leaf area or any other such biological or non-biological object(s).

This software can be used to measure...
  1. Total leaf area
  2. To easily demark senescence and healthy area of the leaf
  3. To study the damage by pathogens
  4. To measure area eaten by insects
  5. To measure variegated area of leaf and floral petals

    This software is useful for agriculture, botany, forestry, entomology, floriculture, and in crop science studies. This software will be useful to measure leaf area of ground nut plants which is one of the major objective of ongoing GSBTM project.
 Research Note: Anther culture – A Promising Area in the field of Plant tissue culture research
Dr. Diwaker R. Patel
Plant Tissue Culture Lab.,
Anand Agriculture University, Anand, INDIA

Anther culture is one of the most extensively investigated areas in rice. Japonicas are easier to culture than indica cultivars. Genotypic specificity and green plant regeneration is a serious problem in indica.
Induction of haploid through anther culture and subsequent doubling of their chromosome number is a rapid method of inbreeding for development of inbreds and fixation of elite recombinants from F1 hybrid.
Rice Anther culture at A.A.U, Anand.

A. Donor plants; B. Mid Uninucleate; C. Anther plating;
D, E. Callus formation from Split anther lobe.
F, G. Green and Albino plant, H. Rooting.
I. Anther Culture raised plants under Hardening.

SOURCE: Plant Tissue Culture Lab., Dept. of Cytogenetics and plant breeding ,B.A.C.A., A.A.U, Anand

Research work on rice anther culture was initiated first time in AAU, Anand using two superior inbreds(Indica group) and a promising hybrid received from Main Rice Research Station, AAU, Nawagam. Plantlets have been obtained successfully via callus followed by invitro rooting. Regenerated plants are under hardening and being studied for gametoclonal variation using cytological and molecular techniques.

  GSBTM is happy to congratulate GBRCP participants for getting admission in PG programmes
VISHALSINGH CHAUDARI
  • Successfully cleared first level of National centre for Biological Sciences (NCBS) entrance exam.
  • Successfully cleared JNU entrance exam with 119th rank.
  • Nirma university biotechnology entrance exam with 11 rank and have joined MS University of Baroda Biotechnology.
SIDDHARTH JAYARAMAN
  • Successfully cleared First level of Indian Institute of Sciences entrance exam.
  • Successfully cleared Nirma University Biochemistry entrance exam with rank 9
NINAD MEHTA
  • Successfully cleared MS University of Baroda Microbiology entrance exam.
  • National Institute of Virology Entrance exam.
  • Nirma University Biochemistry entrance exam with 10th rank
HARSESH BHATT
  • Successfully cleared MS University of Baroda Biochemistry entrance exam.
KINJAL VORA
  • Successfully cleared Pune University MBA Biotech entrance exam.
DATASHA KANTHARIYA
  • Successfully cleared JNU biotechnology entrance exam and joined SP University Industrial Biotechnology.
ALIABBAS SALEH
  • Successfully cleared Nirma University Biotechnology entrance exam with rank 8
MEENA RAJESH MATADEEN
  • Successfully cleared Nirma University Biotechnology entrance exam rank 13
Rine Shah
  • Successfully cleared Nirma University Biochemistry entrance exam
PARESH PUJARA
  • Secured first place in M Sc. Microbiology Gujarat Vidyapith entrance exam
  • Third place in M Sc. Microbiology Ganpat University entrance exam
  • Second place in Ganpat University Biotechnology entrance exam.