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Stem Cell Training

Skill Development in Stem Cell Techniques: Basic Training in Cell Culturing

Stem Cell technology is a technology of hope for patients of many incurable disorders including heart diseases, cancer, HIV, etc. In view of the healthcare solutions offered by stem cells, and its relevance to state of Gujarat, GSBTM is visualizing to develop a platform to enable research, application and use of stem cell technology in the state. The first step towards the same is to generate dedicated skilled manpower in area of stem cell research. Visualizing the thirst and need this GSBTM has organized “Skill Development in Stem Cell Techniques: Basic Training in cell culturing” at GCRI and Stem Cure Pvt. Ltd. in two batches from 19th to 24th December, 2011.

The list of the participant in the stem cell training program:

Sr. No. Name Designation/Department Institution/Company
01. Dr. Rekha R. Iyer Tutor, Pathology Government Medical College, Bhavnagar
02. Dr. Ila N. Hadiyel Assistant Professor, Medicine Government Medical College, Bhavnagar
03. Dr. Sushil K. Suri Professor, Pathology Government Medical College, Bhavnagar
04. Dr. Yogesh Parikh Assistant Professor, Paediatrics Government Medical College, Bhavnagar
05. Dr. Dhaval Parmar Assistant Professor, Forensic Medicine Government Medical College, Bhavnagar
06. Dr. Asruti Kachha Assistant Professor, Pediatrics Government Medical College, Bhavnagar
07. Dr. Seema Baxi Assistant Professor, Pathology Government Medical College, Bhavnagar
08. Dr. S. M. Patel M.D. (Biochemistry), Professor of Biochemistry Government Medical College, Surat
09. Dr. Amith Satasiya Tutor, Pathology B. J. Medical College, Ahmedabad
10. Dr. Parin Parmar Assistant Professor, Paediatrics Pandit Deendayal Upadhyay Medical College, Rajkot
11. Dr. Minesh B. Gandhi Assistant Professor, Pathology Smt. NHLMMC, Ahmedabad (V. S. Hospital)
12. Dr. Imran V. Shaikh Jr. Lecturer, Pathology Smt. NHLMMC, Ahmedabad (V. S. Hospital)
13. Dr. Cherry K. Shah Professor, Pathology Smt. NHLMMC, Ahmedabad (V. S. Hospital)

The training was provided in two premier orgnaisations of Gujarat covering various basic techniques of stem cell culturing.

Gujarat Cancer Research Institute – GCRI

Participants were taught preparation of Media, Handling and maintenance of primary leukemic cells, Isolation of stem cells from dental pulp, Isolation of MNCs from Wharton’s jelly – Explant and Collagenase method, Isolation of Mononuclear cells from bone-marrow, Density gradient separation of Mononuclear cells, Isolation of CD34+ cells from MNCs using Immuno-magnetic cell sorting, Viability check using trypan blue dye counting using hemocytometer.

Stem Cure Pvt. Ltd.

Key techniques in this lab included characterization of stem cells via Gene-expression studies by isolating RNA and amplification of cDNA by RT- PCR, Stem cell culture and sub-culture techniques, passaging and disaggregation using trypsin / collagenase, Techniques for cell-count, viability check and planting of stem cells, Cryopreservation techniques such as freezing, thawing and media change of ongoing stem cells.

Trainees were given hads on exposure to these basic techniques along with the relevant theory portion. This traininng program has facilitated development of skills in the medicos and professional of Surat Medical College and V.S. Hospital Ahmedabad where soon Govenemnt is establishing Stem Cell Therapeutics Units.

Skill Development in Stem Cell Techniques: Basic Training in Cell Culturing Skill Development in Stem Cell Techniques: Basic Training in Cell Culturing Skill Development in Stem Cell Techniques: Basic Training in Cell Culturing

Page last updated on: 11/3/2014

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